The Cardiovascular
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Peripheral Blood Stem Cell Collection (CD34+ Yield)

Predicted CD34+ cell yield per kilogram from a leukapheresis procedure.

Peripheral Blood Stem Cell Collection (CD34+ Yield)
Pre-apheresis peripheral blood CD34+ count
cells/uL
Blood volume to be processed
L
Patient weight
Collection efficiency
Fraction of the CD34+ cells passing through the separator that are actually collected; typically 40-70%.
%
Complete the fields above to see the result.

When to use

  • Planning apheresis volume/duration and predicting whether a collection will meet transplant-dose targets.

Formula

Predicted yield (x10^6 CD34+ cells/kg) = pre-apheresis CD34+ count (cells/uL) x blood volume processed (L) x collection efficiency (fraction) / patient weight (kg).

Pearls and pitfalls

  • A minimum of 2 x10^6 CD34+ cells/kg is generally required for autologous engraftment; 5 x10^6/kg is often used as an optimal target.

References

  1. Weaver CH, et al. Blood. 1995;86(10):3961-3969 (CD34+ dose and engraftment kinetics).
  2. Schlenke P, et al. Vox Sang. 2000;78(3):185-192 (collection efficiency formula).